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KMID : 0381920070370010001
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Korean Journal of Microscopy
2007 Volume.37 No. 1 p.1 ~ p.10
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Effect of Haedokjeongki-tang in Rat Liver after Monocrotaline Exposure
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Park Hyun-Jung
Kim Jeong-Sang
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Abstract
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Monocrotaline (MCT) is a pyrrolizidine alkaloid (PA) plant toxin that produces hapatotoxicity in humans and animals. To test the hypothesis, we investigated the possible protective effects of Haedokjeongki-tang as an antioxidant against MCT-induced liver injury in rats. Cells with apoptotic morphology have been observed in the livers of animals exposed to PA and Haedokjeongki-tang. Whether apoptosis occurs in the livers of MCT-treated animals and whether it is required for full manifestation of pathological changes is not known, To determine this, rats were treated with 100 mg MCT/kg, and apoptosis was detected by transmission electron microscopy and TUNEL assay. MCT produced apoptosis in the liver by 6 h after treatment and increased by 24 h. Administration of Haedokjeongki-tang did affect liver structure and inhibit apopotosis in MCT-induced liver injury. Upon light and electron microscopic examination, we observed that aedokjeongki-tang improved the morphological and histopathological changes of the liver caused by MCT-induced injury. MCT caused a timedependent release of GOT and GPT, a marker of liver injury. Furthermore, we observed with respect to antioxidants status, catalase and superoxide dismutase activity tended to be higher in the MCT-treated rats than in the Haedokjeongki-tang administered rats. Our finding showed that Haedokjeongki-tang administration partially reduced liver injury after MCT exposure.
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KEYWORD
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Haedokjeongki-tang, Liver injury, MCT, PA, Rat
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